cell signaling cat 3177  (Cell Signaling Technology Inc)

Journal: BMC Research Notes

Article Title: Fagopyrum dibotrys extract improves nonalcoholic fatty liver disease via inhibition of lipogenesis and endoplasmic reticulum stress in high-fat diet-fed mice

doi: 10.1186/s13104-024-06962-x

Figure Lengend Snippet: FDE attenuates hepatic ER stress and inhibits activation of PERK pathway in HFD-fed mice. ( A ) Representative images of immunofluorescence staining for GRP78 in the livers of mice (magnification 200× and 400×; scale bar, 50 μm and 25 μm). ( B ) Quantification of GRP78 fluorescence intensity. ( C ) Protein expression of GRP78 and CHOP determined by Western blot. ( D , E ) Quantification of protein expression. ( F ) Protein expression of P-EIF2α and EIF2α determined by Western blot. ( G ) Quantification of protein expression. Control, normal diet group; Control + FDE, normal diet + Fagopyrum dibotrys extract; HFD, high-fat diet group; HFD + FDE, high-fat diet + Fagopyrum dibotrys extract. The presented data represent the mean ± SEM ( n = 6). ** p < 0.01; *** P < 0.001; **** P < 0.0001

Article Snippet: The slices were hydrated in gradient alcohol, washed with PBS three times, blocked with goat serum to prevent nonspecific antibody binding, and then incubated overnight at 4 °C with specific primary antibodies against GRP78 (Cell Signaling Technology, Cat#3177, USA; 1:200).

Techniques: Activation Assay, Immunofluorescence, Staining, Fluorescence, Expressing, Western Blot, Control

Journal: Cell reports

Article Title: KDEL Receptors Are Differentially Regulated to Maintain the ER Proteome under Calcium Deficiency

doi: 10.1016/j.celrep.2018.10.055

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: rabbit anti-BiP , Cell Signaling , Cat3177S; RRID:AB_2119845.

Techniques: Virus, Recombinant, Modification, Blocking Assay, Plasmid Preparation, cDNA Synthesis, DC Protein Assay, Proliferation Assay, MTS Assay, Cell Viability Assay, ATP Assay, Software, Imaging, Mass Spectrometry, Chromatography